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Chromatography__

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Chromatography__

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it's aumsum time

27 Views • Oct 26, 2019

Wikipedia Context

In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent called the mobile phase, which carries it through a system on which a material called the stationary phase is fixed. As the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.

Full Article

Description

Our topic for today is Chromatography.
Does black ink consist of only black color or any other color as well?
Let us find out by the process of paper chromatography.
Chromatography is a technique of separating two or more dissolved solids which are present in a solution in very small quantities.
Take a strip of filter paper.
Draw a line with a pencil close to the lower edge of the paper.
Put a small drop of ink on the center of the line.
Let it dry for sometime.
Take a jar containing some water.
Put the filter paper in it such that the drop of ink is just above the water level.
After sometime, the water starts gradually rising up by capillary action.
As the water moves up, it takes the dyes present in the ink along with it.
Slowly, we start seeing some different colors on the paper as the water rises up, these are the different dyes present in the ink.
Different dyes have different solubility levels and travel at different speeds as the water rises up.
As a result, we see different colors at different heights and the dyes get separated.
Hence, in this way paper chromatography is used to separate the different colors in a dye.